Comparing with human body PET imaging, the small animal PET imaging requires higher spatial resolution and sensitivity, thus it has promoted the development of scintillation array preparation process. LYSO crystal is considered to be the best scintillator nowadays with most comprehensive properties，including rapid responding time, high density and effective atomic number etc. This paper proposes a production process of LYSO scintillator array which can achieve large size LYSO scintillation crystal growth using Czochralski method and polish the crystal pieces by utilizing chemical mechanical polishing method. Ultimately the LYSO scintillator array for small animal PET applications can be manufactured by using the homemade fixture with high precision.