The effects of three kinds of commonly used sensitive gases of dry air, nitrogen (N2) and helium (He) on the sensitivity of the fluidic gyroscope have been studied in this work. By using the finite element method, the flow field in the sensing element of fluidic gyroscope under the effect of the input angular velocity (ωi) of 20 (°)/s was calculated The calculated results and experiment have shown that the distribution of flow field changes by changing the type of sensitive working gas in the fluidic gyroscope sensitive element. The input angular velocity (ωi) is 20 (°)/s, the airflow velocity difference between two heat resistance wire r1 and r2 namely ΔvN2＞ΔvAIR＞ΔvHe. the sensitivity coefficientKN2＞KAIR＞KHe, among them, KN2 is 1.05 times ofKAIR,KHe, is 0.21％of KAIR. Nitrogen is corresponding to the highest sensitivity, the thermal resistance wires antioxidant, stability is better, but the cost is high; sensitivity of dry air secondly, the thermal resistance wires easily oxidized, poor stability; helium corresponding to the minimum sensitivity, thermal resistance wire is not easy to be oxidized, the best stability. This paper explained the mechanism of sensitive working gas influence on fluidic gyroscope sensitivity, and a new way was opened up for improving the practicability of the fluidic gyroscope to meet different engineering needs.