A novel UHF RFID reader is designed based on Impinj Indy R2000 chip, and the AT91SAM7S256 microcontroller chips are used as the digital processing module. The operating frequency of the system is 860~960 MHz and the RF output power is up to 30 dBm by using the external power amplifier module. The proposed splitter isolation circuit has the features of simple structure, easetointegrate and high isolation. The design of the main modules of the reader are introduced, the measurement results of the key performance indicators are given and the feasibility of the system design is verified.