Aiming at the problem for the analysis of the dynamic characteristics of piezo-driven fatigue testing machine, a kinetic model of the piezoelectric fatigue test system was established based on the vibration mechanics theory.The natural frequencies and the dynamic load expression was obtained. The efffects of the stiffness of the piezoelectric vibration natural frequency and the dynamic loads were analyzed through the matlab simulation. Finally, the experimental test was carried out and the results showed that the obtained experimental data and theoretical analysis were agreed well within the error range, and the validity of the theoretical analysis was verified. The study provided a theoretical basis for tracking the piezoelectric driven system resonance frequency and controlling the work load precision, and the dynamic performance of the piezoelectric-driven fatigue test systems was predicted, thus has a certain value in engineering.