For the demand of micro/nano manipulator in the biomedical engineering, a kind of microoperation platform which can perform multiple operations on the biological tiny cells is proposed in this paper. The platform is consists of a 3DOF precision positioning instrument, negative pressure drive unit (the V type linear ultrasonic motor, syringe and silicone hose) and the nanometer through hole based on glass. The accuracy of the positioning instrument is 1 μm, the instrument is easy to operate and has high positioning efficiency. The specific biological cells can be adsorbed and controlled precisely by the absorption force which is generated in the negative pressure drive unit. The external diameter of the nanometer through hole is less than 50 μm which can operate on more tiny biological cells. The independently designed and manufactured V type linear ultrasonic motor and the nanometer through hole are used as the key components in the platform for the first time. The experiments on the biological cell manipulation show that the positioning, adsorption, release and movement of biological cells are carried out finally in the operation platform. The size of the operated cells is less than 20 μm.