The CdTe quantum dots (QDs)based DNA electrochemiluminescence (ECL) sensor was prepared for rapid and sensitive detection of the specific gene sequence. The proposed sensor used the gold electrode as the substrate,the Lcysteine and the probe of singlestranded DNA(ssDNA) were bonded successively. The assembly process of sensor was characterized by the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The target ssDNA labeled by CdTe QDs and the probe ssDNA were hybridized to form an ECL sensing system. The result indicated that the optimum electrochemiluminescence performance was obtained at the scan time of 6 s,scan potential of -3.0~-1.5 V and scan rate of 0.1 V/s.