A 16channel programmable charge amplifier board card for the structural health monitoring system based on the piezoelectric sensor was specially designed in this paper. The functions and working states controls of the module were carried out by the embedded microcontroller chip named STM32F407IGT6. The programmable charge amplifier board card includes a drive sensor selection module, a charge conversion module, a passive filter module, a twostage amplifier and trimming module, a bandpass filter module, a USB transmission module, etc. These modules can satisfy the various functions of the developed charge amplifier and have the advantages of small size, high integration and so on. The board card can communicate with a PC through the USB serial port, while the user interface in the PC was developed based on the LabVIEW software.