The drift of fiber optic gyroscope in inertial navigation system is significantly affected by temperature changes, which leads to various restrictions on its application in navigation systems. In this paper, a software temperature compensation method is proposed, and a multiparameter polynomial temperature compensation model based on the fiber optic gyro is established, and the corresponding test method for the temperature compensation of gyroscope drift is designed. The experiment show that the proposed temperature model is accurate and effective, which can compensate for the drift of optical fiber gyroscope caused by temperature changes, so as to improve the navigation accuracy and temperature adaptability of the system.