The transporting characteristics of microdroplets along the spherical surface driven by surface acoustic wave were investigated. The bottom of the steel ball was adhered to the 128° YX-LiNbO3 piezoelectric substrate by polydimethylsiloxane, and is cured in an incubator. The microdroplets on the piezoelectric substrate were transported along the surface of the ball under the combined action of the surface acoustic wave driving force and the ball resistance. For convenience of observation, the transporting characteristics of microdroplets on spherical surface under surface acoustic waves were studied using the red ink aqueous solution as experimental object. The results showed that the transport properties of microdroplets on spherical surfaces were determined by the electrical signal power which excited the surface acoustic wave, the diameter of the spherical ball and the volume of the microdroplets. The microdroplets of 3 μL can be transported to the top of the steel ball with a diameter of 2 mm at the electrical signal power of 28.7 dBm.