In view of the high dependence of the traditional accelerometer calibration method on high precision turntable, an optimized nine position calibration algorithm based on MEMS accelerometer is proposed in this paper.The nonlinear factor and electronic crosstalk effect of the accelerometer are taken into consideration, and by collecting the static accelerometer output data of nine positions, the zero bias, scale factor and installation error of the accelerometer can be quickly and accurately calibrated, for which the dependency on the traditional turntable is eliminated. The experimental results show that the nonlinear problem of accelerometer has been effectively solved by the nineposition calibration algorithm based on MEMS accelerometer and the influence of electronic crosstalk on the accelerometer has been reduced.The error accuracy of accelerometer is reduced from 8.48×10-3g(g=9.8 m/s2) to 5.1×10-4g, which proves the feasibility and effectiveness of the nineposition calibration algorithm.