In order to reduce the process requirements and manufacturing costs, a method for fabricating the paperbased microfluidic device on a piezoelectric substrate is proposed. The microchannel patterns are formed by the soldering tin wire, and then transferred to the paper substrate by a series of processes of curing by polydimethylsiloxane (PDMS), lifting off the soldering tin wire, filling the cotton thread, and absorbing the uncured PDMS by the cotton thread, and bonding the pattern on 128°YXLiNbO3 piezoelectric substrate. The experimental results show that the color development reaction of the starch is implemented in the paperbased microfluidic device at 27.3 dBm signal power, which verifies the microfluidic analysis function.