Aiming at the problems of low control precision and weak antiinterference ability of PI controlled micronano manipulation imaging system, a method based on linear autodisturbance rejection control (LADRC) is proposed. First, the structure and mathematical model of the micronano manipulation imaging system are analyzed, then a linear autodisturbance rejection controller is designed, and the partial parameters of the linear extended state observer with the model information as well as the parameters of the PI controller are determined by using the obtained parameters of the model. Finally, a controller model was established in Matlab/Simulink and the simulation experiment comparison was carried out. The results show that compared with the traditional PI control, this study has the characteristics of high control precision and strong antidisturbance ability, and effectively improves the performance of the system.